Peptidylarginine deiminase type 2 is over expressed in the glaucomatous optic nerve
نویسندگان
چکیده
PURPOSE To determine levels of Peptidyl arginine deiminase 2 (PAD2) and its product protein-bound citrulline in cadaver eyes that suffered from normal tension glaucoma (NTG) compared to primary open angle glaucoma (POAG), and controls. METHODS Western analysis, ELISA, and immunohistochemical analysis were performed with human tissues. RESULTS We report over expression of PAD2 and higher levels of its product protein-bound citrulline in the optic nerve of normal tension glaucoma patients (NTG). CONCLUSIONS This is the first report demonstrating that like in POAG, NTG also possesses elevated levels of both PAD2 and protein-bound citrulline.
منابع مشابه
PAD2 – peptidylarginine deiminase type 2; RT-PCR – reverse transcriptase-polymerase chain reaction; SDS – sodium dodecyl sulfate Short communication MICROGLIAL EXPRESSION OF PEPTIDYLARGININE DEIMINASE 2 IN THE PRENATAL RAT BRAIN
Peptidylarginine deiminases (PADs) are Ca-dependant posttranslational modification enzymes that catalyze the citrullination of protein arginyl residues. PAD type 2 (PAD2) is thought to be involved in some processes of neurodegeneration and myelination in the central nervous system. In this study, we found PAD2-positive cells in rat cerebra in 19to 21-day old embryos, i.e. at a developmental sta...
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